赵建军;高晓娟;王英华;马玲;王汉卿;
ZHAO Jian-jun;GAO Xiao-juan;WANG Ying-hua;MA Ling;WANG Han-qing;College of Pharmacy,Ningxia Medical University;Ningxia Institute for Drug Control;Ningxia Research Center of Modern Hui Medicine Engineering and Technology,Ningxia Medical University;

• 1:宁夏医科大学药学院
• 2:宁夏回族自治区药品检验所
• 3:宁夏医科大学宁夏回药现代化工程技术研究中心

摘要(Abstract)：

黄芩为我国常用大宗中药材,其野生资源急剧减少,目前我国宁夏、河北、山西、甘肃等地大面积栽培,供中药材市场及临床应用。该研究旨在通过12批栽培黄芩药材和黄芩对照药材的HPLC指纹图谱及近红外图谱研究,对4个产区的栽培黄芩药材进行质量评价。结果显示,12批栽培黄芩药材与黄芩对照药材色谱指纹图谱和近红外图谱的相似度分别大于0.961和0.983,结合12批栽培黄芩药材与黄芩对照药材共有峰面积(近红外为对应点吸收值)的配对样本t检验,均无显著差异,验证了12批栽培黄芩药材与对照药材质量的一致性较高。该研究基于色谱指纹图谱与近红外图谱,并结合配对样本t检验对分析结果进行验证,避免了分析中因指纹图谱相似度阈值限定不明确而引起的误判,为分析结果提供了可靠的依据。同时,通过对综合性较强的2种检测手段的比较研究,为完善中药材质量控制方法提供了新的思路。
Scutellaria baicalensis is a common and important medicinal plant in China,facing with reducing sharply in wild resources. To meet the needs in Chinese herbwouls medicine market and clinical application,S. baicalensis has been widely cultivated in Ningxia,Hebei,Shanxi,and Gansu et al. HPLC finger-print and near-infrared were studied in the research to evaluate quality difference of S. baicalensis in four districts. The results showed that the similarity of HPLC finger-print of 12 cultivated S. baicalensis and reference crude herb is more than 0. 961,and the other is more than 0. 983. On the other hand,paired sample t-test indicated there has no significant difference between the common peaks' area of 12 cultivated S. baicalensis and reference crude herb. It was verified that 12 cultivated S. baicalensis has highly consistency with reference crude herb. On the basis of chromatographic finger-print and near-infrared spectrum,the study applied paired sample t-test to verify analysis results,which could avoid erroneous judgment induced by indefinite threshold values in the similarity of chromatographic finger-print and provide reliable basis for the analysis results. Meanwhile,it also provides a new idea for improving the quality control method of Chinese medicinal materials by comparative study about two comprehensive detection means.

关键词(KeyWords)： 黄芩;指纹图谱;近红外;化学成分;质量控制
Scutellaria baicalensis;fingerprint;near-infrared spectra;chemical composition;quality control

Abstract:

Keywords:

基金项目(Foundation): 宁夏科技支撑计划项目(2015BY032)

作者(Author): 赵建军;高晓娟;王英华;马玲;王汉卿;
ZHAO Jian-jun;GAO Xiao-juan;WANG Ying-hua;MA Ling;WANG Han-qing;College of Pharmacy,Ningxia Medical University;Ningxia Institute for Drug Control;Ningxia Research Center of Modern Hui Medicine Engineering and Technology,Ningxia Medical University;

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DOI:

参考文献(References)：

• [1]中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1977.
• [2]中国药典.一部[S].2015:301.
• [3]王丹,蒋亚杰,梁艳,等.黄芩不同规格与化学成分及内在质量相关性的研究[J].中国中药杂志,2012,37(4):426.
• [4]施高翔,邵菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713.
• [5]柴玉爽,雷帆,邢东明,等.黄芩苷对模式识别受体TLR2/4-NOD2的作用及其成药性意义[J].中国中药杂志,2013,38(16):2639.
• [6]Yuan Q J,Zhang Z Y,Hu J,et al.Impacts of recent cultivation on genetic diversity pattern of a medicinal plant,Scutellaria baicalensis[J].Bmc Genetics,2009,11(1):52.
• [7]梁逸曾,易伦朝,黄熙,等.中药分析新思路及其质量控制[J].分析测试学报,2014,33(2):119.
• [8]黄洋,邵慧凯,路丽,等.不同采收期小叶榕叶HPLC指纹图谱研究[J].中草药,2014,45(2):271.
• [9]邵林,郭庆梅,冉蓉,等.山东不同栽培品种金银花HPLC指纹图谱的比较[J].中国实验方剂学杂志,2011(19):117.
• [10]詹雪艳,史新元,段天璇,等.色谱指纹图谱相似度方法的研究进展[J].中国实验方剂学杂志,2011,17(2):248.
• [11]Roggo Y,Chalus P,Maurer L,et al.A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies[J].J Pharm Biomed Anal,2007,44(3):683.
• [12]陈林伟,秦昆明,徐雪松,等.中药指纹图谱数据库的研究现状及展望[J].中草药,2014,45(21):3041.
• [13]袁明洋,黄必胜,余驰,等.8种含碳酸盐的矿物类中药近红外定性定量模型的建立[J].中国中药杂志,2014,39(2):267.
• [14]汪小莉,李嬛,秦昆明,等.近红外光谱学与化学计量学在中成药液体制剂过程分析中的应用[J].中草药,2013,44(15):3420.