中国中药杂志

2021, v.46(06) 1460-1466

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人参皂苷Rg_1对心肌细胞缺氧/复氧损伤的保护作用及其机制研究
Protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation injury and its mechanism

李千会;葛卓望;田丁;相银;陈昱;张亚臣;
LI Qian-hui;GE Zhuo-wang;TIAN Ding;XIANG Yin;CHEN Yu;ZHANG Ya-chen;Department of Geriatrics,Zhongda Hospital,Southeast University;Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University;Department of Cardiology,Sixth People′s Hospital,Shanghai Jiaotong University;

摘要(Abstract):

探讨人参皂苷Rg_1对H9c2心肌细胞缺氧/复氧(hypoxia/reoxygenation, H/R)损伤的保护作用及其信号通路。建立H9c2心肌细胞的H/R模型,分成不同的处理组,利用CCK-8(cell counting kit-8)法检测心肌细胞的活性,Brdu法检测H9c2细胞的增殖,检测心肌细胞caspase-3的活性,Western blot法检测蛋白表达。利用流式细胞仪检测心肌细胞凋亡水平。人参皂苷Rg_1可抑制H/R导致的心肌细胞凋亡和caspase-3的活性;可促进核因子红细胞2相关因子2 (nuclear factor erythroid-2 related factor 2,Nrf2)核转录,同时可增强下游基因血红素加氧酶1(heme oxygenase-1,HO-1)表达;可随着浓度的增加(10,20,40,60μmol·L~(-1)) Nrf2核转录和HO-1表达增加;然而,心肌细胞转染Nrf2-siRNA后人参皂苷Rg_1保护心肌细胞的作用明显减弱。人参皂苷Rg_1可通过激活Nrf2/HO-1信号通路保护心肌细胞。
This project aimed to explore the protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation(H/R)-induced H9 c2 cardiomyocyte injury and its underlying signaling pathway. The H/R model of H9 c2 cardiomyocytes was established and then the cells were divided into different treatment groups. CCK-8(cell counting kit-8) was used to detect the activity of cardiomyocytes; Brdu assay was used to detect the proliferation of H9 c2 cells; the caspase-3 activity was tested, and then the protein expression was assessed by Western blot. Flow cytometry was used to evaluate the apoptosis level of cardiomyocytes. Ginsenoside Rg_1 inhibited H/R-induced cardiomyocyte apoptosis and caspase-3 activity, promoted nuclear transcription of nuclear factor erythroid-2 related factor 2(Nrf2), and enhanced the expression of the downstream heme oxygenase-1(HO-1). Ginsenoside Rg_1 could increase Nrf2 nuclear transcription and HO-1 expression with the increase of concentration(10, 20, 40, 60 μmol·L~(-1)). However, the protective effect of ginsenoside Rg_1 on cardiomyocytes was significantly weakened after the transfection of Nrf2-siRNA. Ginsenoside Rg_1 could protect cardiomyocytes by activating the Nrf2/HO-1 pathway.

关键词(KeyWords): 人参皂苷Rg_1;缺氧/复氧;缺血再灌注损伤;凋亡
ginsenoside Rg_1;hypoxia/reoxygenation;ischemia-reperfusion injury;apoptosis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81770242)

作者(Author): 李千会;葛卓望;田丁;相银;陈昱;张亚臣;
LI Qian-hui;GE Zhuo-wang;TIAN Ding;XIANG Yin;CHEN Yu;ZHANG Ya-chen;Department of Geriatrics,Zhongda Hospital,Southeast University;Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University;Department of Cardiology,Sixth People′s Hospital,Shanghai Jiaotong University;

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DOI: 10.19540/j.cnki.cjcmm.20201123.401

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