中国中药杂志

1998, (12) 3-5

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哈帕甙在魔鬼爪浸膏和帕歌斯风湿乐片中的薄层鉴别及薄层扫描含量测定方法研究
TLC Analysis of Harpagoside in Devil Claw Extract and Its Tablets

钱浩泉,谢培山
Qian Haoquan and Xie Peishan (Guangzhou Municipal Institute for Drug Control,Guangzhou 510160)

摘要(Abstract):

目的:对魔鬼爪浸膏(Devil’sClawExtract)及制剂帕歌斯风湿乐片(PagosidDevil’sClawExtractTablets)中的环烯醚萜甙类成分进行定性鉴别,并定量测定其主要活性成分哈帕甙(harpagoside)。方法:硅胶预制薄层板,氯仿乙酸乙酯甲醇水为展开剂,5%香草醛硫酸溶液或10%硫酸乙醇溶液为显色剂做为定性鉴别;并采用薄层扫描法测定哈帕甙的含量,以氢氧化钠碱性板展开后,于283nm紫外吸收扫描。结果:线性范围16.8ng~151.2ng,平均回收率为101.2%。结论:本方法经线性关系、灵敏度、专属性、稳定性及重现性方法考察证明符合定量要求。
Objective:To set up a method for TLC identification of the Devil Claw Extract and its tablets,and make a quantitative analysis of harpagoside the main active constituent in the Extract.Method:A characteristic TLC chromatogram was obtained by using pre coated silica gel 60F 254 plate,chloroform ethyl acetate methanol water as the solvent system and 5% vanillin sulfuric acid solution or 10% sulfuric acid ethanol solution as the spray reagent;and quantitative analysis was carried out using silica gel plate modified by 1% sodium hydroxide,and UV absorptive scannig at 283nm.Result:The regression equation is Y=20.33X+92.72,r=0.9993,linear range between 16.8~151.2ng,and average recovery 101.2%.Conclusion:This method has been validated by linearity,stability,sensitivity and reproducibility tests,and proved to be accordant with the requirement for quantitative analysis of the herbal medicine in question.

关键词(KeyWords): 魔鬼爪浸膏,帕歌斯风湿乐片,哈帕甙,薄层鉴别,薄层扫描
Devil Claw Extract;Pagosidel Devil Claw Extract tablets;harpagoside;TLC;TLC scanning

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作者(Author): 钱浩泉,谢培山
Qian Haoquan and Xie Peishan (Guangzhou Municipal Institute for Drug Control,Guangzhou 510160)

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