中国中药杂志

2013, v.38(06) 856-860

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草珊瑚药材HPLC特征指纹图谱研究
HPLC characteristic chromatographic profile of Sarcandra glabra

林培玲;曾建伟;丁春花;梁一池;
LIN Pei-ling1,ZENG Jian-wei2,DING Chun-hua1,LIANG Yi-chi1*(1.College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 2.Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

摘要(Abstract):

目的:建立草珊瑚药材HPLC特征指纹图谱分析方法,为其质量控制提供依据。方法:采用Agilent ZorbaxEclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-甲醇(1∶9)-0.5%甲酸溶液梯度洗脱;检测波长344 nm;柱温40℃。结果:建立了草珊瑚药材HPLC特征指纹图谱共用模式,标定了20个共有峰,并指认了7个主要色谱峰。通过主成分聚类分析,46个不同产地草珊瑚药材可分为5类。结论:该方法快速,可较全面的反映草珊瑚药材中化学成分的信息,为草珊瑚药材的质量控制提供了科学实验依据。
Objective: To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.Method: The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) with column temperature at 40 ℃.The mobile phase was consisted of water containing 0.5% formic acid and acetonitrile to methanol(1∶9) in gradient mode,and the detection wavelength was set at 344 nm.Result: A common mode of the HPLC characteristic chromatographic profile has been establised.There were 20 common peaks,seven of which were identified,and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.Conclusion: The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S.glabra.

关键词(KeyWords): 草珊瑚;HPLC;指纹图谱;主成分聚类分析
Sarcandra glabra;HPLC;characteristic chromatographic profile;principal component cluster analysis

Abstract:

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基金项目(Foundation): 福建省科技厅重点项目(2011Y4005,2011Y3008)

作者(Author): 林培玲;曾建伟;丁春花;梁一池;
LIN Pei-ling1,ZENG Jian-wei2,DING Chun-hua1,LIANG Yi-chi1*(1.College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 2.Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

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