张君毅;郭巧生;吴丽伟;杭悦宇;
ZHANG Jun-yi~(1),GUO Qiao-sheng~(1),WU Li-wei~(1),HANG Yue-yu~(2)(1.Institute of Chinese Medicinal Materials,Nanjing Agriculture University,Nanjing 210095,China;2.Jiangsu Province and Chinese Academy of Science,Nanjing 210014,China)

• 1:南京农业大学中药材研究所
• 2:南京农业大学中药材研究所
• 3:南京农业大学中药材研究所
• 4:江苏省中国科学院植物研究所 江苏南京210095

摘要(Abstract)：

目的:研究我国不同地区半夏核糖体ITS碱基序列差异及其与其地理分布和外部形态的相关性。方法:运用PCR法对半夏ITS1-5.8S-ITS2序列扩增后直接测序,用软件CLUSTRAL 1.83和MEGA 3.1分析测序结果。结果:得到我国半夏主要的16个居群18个样本rDNA中的ITS和5.8S rDNA完全序列。ITS1,5.8S和ITS2序列长度分别为276,162,246 bp。ITS2碱基频率差异显著,ITS1较为保守。根据两者序列以邻接法建立分子系统发生树。结论:半夏rDNA变异与其地理分布相关,与其外部形态关系有待进一步研究。
Objective: To study the ITS sequence variation of Pinellia ternata from different population in China,and it correlation to geographical distribution and morpha of the plant.Method: The rDNA ITS regions of various P.ternata were amplified and sequenced.And they were analyzed by means of the software of CLUSTRAL and MEGA.Result: Complete sequence of ITS and 5.8S rDNA of 16 different P.ternata population were obtained.The sequences of ITS1,5.8S and ITS2 are 276,162 and 246 bp,respectively.ITS1 was more conservative than ITS2.Phylogenetic tree based on ITS1 and ITS2 sequences data was conducted by Neighbor-joining method.Conclusion: Ribosomal DNA ITS sequence analyses can be applied to the resource research of P.ternata.

关键词(KeyWords)： 半夏;rDNA;ITS;鉴别;珠芽
Pinellia ternata;ribosomal DNA;ITS;authentication

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30070922);; 江苏省自然科学基金资助项目(前期预研)(BK2005206)

作者(Author): 张君毅;郭巧生;吴丽伟;杭悦宇;
ZHANG Jun-yi~(1),GUO Qiao-sheng~(1),WU Li-wei~(1),HANG Yue-yu~(2)(1.Institute of Chinese Medicinal Materials,Nanjing Agriculture University,Nanjing 210095,China;2.Jiangsu Province and Chinese Academy of Science,Nanjing 210014,China)

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